• 我想一个解决方案，能够打印约100 pdf文件，每天每约200页长。文件存储在特定的目录。*

import win32api
import win32print
from glob import glob

# A List containing the system printers
all_printers = [printer[2] for printer in win32print.EnumPrinters(2)]
# Ask the user to select a printer
printer_num = int(input("Choose a printer:\n"+"\n".join([f"{n} {p}" for n, p in enumerate(all_printers)])+"\n"))
# set the default printer
win32print.SetDefaultPrinter(all_printers[printer_num])
pdf_dir = "D:/path/to/pdf_dir/**/*"
for f in glob(pdf_dir, recursive=True):
    win32api.ShellExecute(0, "print", f, None,  ".",  0)

input("press any key to exit")

备注：
1.您可以使用DevMode对象（GUI代码示例）更改打印机属性，如彩色/黑色、缩放比例、质量等。
1.提供文件名的绝对路径
1.退出脚本之前，请给予足够的时间来处理作业。
虽然不能直接回答这个问题，但我还是忍不住用tkinter制作了一个GUI。它可以很容易地更改为选择一个目录--以及其中的所有文件--而不是一个文件。

import win32api
import win32print
import traceback

from tkinter.filedialog import askopenfilename
from tkinter import *
from tkinter import font # * doesn't import font or messagebox
from tkinter import messagebox

root = Tk()
root.title("Python Printer")
root.geometry("410x310")
root.resizable(False, False)
root.tk.call('encoding', 'system', 'utf-8')

def font_size(fs):
    return font.Font(family='Helvetica', size=fs, weight='bold')

# Add a grid
mainframe = Frame(root)
#mainframe.grid(column=0,row=0, sticky=(N,W,E,S) )
mainframe.grid(column=0,row=0, sticky=(N) )
mainframe.columnconfigure(0, weight = 1)
mainframe.rowconfigure(0, weight = 1)
mainframe.pack(pady = 10, padx = 0)

# Create a _printer variable
_printer = StringVar(root)
# Create a _color variable
_color = StringVar(root)
_filename = ""

# on change dropdown value
def sel_printer(*args):
    print( _printer.get() )
# link function to change dropdown
_printer.trace('w', sel_printer)

def sel_color(*args):
    print( _color.get() )
# link function to change dropdown
_color.trace('w', sel_color)

def UploadAction(event=None):
    global _filename
    _filename = filedialog.askopenfilename()
    #print('Selected:', _filename)

def PrintAction(event=None):

    PRINTER_DEFAULTS = {"DesiredAccess":win32print.PRINTER_ALL_ACCESS} 
    pHandle = win32print.OpenPrinter(_printer.get(), PRINTER_DEFAULTS)
    properties = win32print.GetPrinter(pHandle, 2)
    properties['pDevMode'].Color = 1 if str(_color.get()) == "Color" else 2
    properties['pDevMode'].Copies = 1
    win32print.SetPrinter(pHandle, 2, properties, 0)

    if not _filename:
        messagebox.showerror("Error", "No File Selected")
        return
    elif not _printer.get():
        messagebox.showerror("Error", "No Printer Selected")
        return

    try:
        #win32print.SetDefaultPrinter(_printer.get())
        win32api.ShellExecute(0, "print", _filename, None,  ".",  0)
        win32print.ClosePrinter(pHandle)
    except:
        pass
        messagebox.showerror("Error", "There was an error printing the file :(")

choices = [printer[2] for printer in win32print.EnumPrinters(2)]
_printer.set(win32print.GetDefaultPrinter()) # set the default option

popupMenu = OptionMenu(mainframe, _printer, *choices)
popupMenu['font'] = font_size(12)
Label(mainframe, text="SELECT PRINTER").grid(row = 1, column = 1)
popupMenu.grid(row = 2, column =1)

# Dictionary with options
choices = ["COLOR", "MONOCHROME"]
_color.set("COLOR") # set the default option

popupMenu2 = OptionMenu(mainframe, _color, *choices)
popupMenu2['font'] = font_size(12)
Label(mainframe, text="COLOR MODE").grid(row = 3, column = 1)
popupMenu2.grid(row = 4, column =1)

Label(mainframe, text="SELECT FILE").grid(row = 5, column = 1)
button = Button(mainframe, text=u"\uD83D\uDCC1" ' BROWSE', command=UploadAction)
button['font'] = font_size(12)
button.grid(row = 6, column =1)

_copies = IntVar()
_copies.set(1)

def copies_increase(event=None):
    _copies.set(_copies.get() + 1)

def copies_decrease(event=None):
    _copies.set(_copies.get() - 1)
    if _copies.get() < 1 :
        _copies.set(1)

Label(mainframe, textvariable=_copies).grid(columnspan=2)
button_frame = Frame(mainframe)
button_frame.grid(columnspan=2)

dec_button = Button(button_frame, text=u"\u2212", command=copies_decrease, fg="dark green", bg = "white", height=1, width=3 )
dec_button['font'] = font_size(10)

inc_button = Button(button_frame, text=u"\uFF0B", command=copies_increase, fg="dark green", bg = "white", height=1, width=3 )
inc_button['font'] = font_size(10)

button_frame.columnconfigure(0, weight=1)
button_frame.columnconfigure(1, weight=1)

dec_button.grid(row=0, column=0, sticky=W+E)
inc_button.grid(row=0, column=1, sticky=W+E)

Label(mainframe).grid(row = 10, column = 1)
p_button = Button(mainframe, text=u'\uD83D\uDDB6' + " PRINT", command=PrintAction, fg="dark green", bg = "white")
p_button['font'] = font_size(18)
p_button.grid(row = 11, column =1)

root.mainloop()

GIST
第一次
来源：

1. Python printing a pdf file on my Brother Laser printer (Duplex print on/off)
2. Python打印pdf文件与win32 print
3. http://docs.activestate.com/activepython/3.1/pywin32/PyDEVMODE.html
4. https://www.thecodingforums.com/threads/printing-using-python.502484/
5. https://stackoverflow.com/a/37586129/797495
6. https://stackoverflow.com/a/56638762/797495
7. https://t.codebug.vip/questions-712908.htm
8. How do I create a button in Python Tkinter to increase integer variable by 1 and display that variable?
9. https://gregcaporale.wordpress.com/2012/01/18/powershell-to-print-files-automatically/（这也可能是一种解决方案）
   PS：我可能不会赢得奖金，但我确实很喜欢制作GUI。tkinter布局是最难的部分！

这是我用过的代码，它工作正常。

name = win32print.GetDefaultPrinter() # verify that it matches with the name of your printer
printdefaults = {"DesiredAccess": win32print.PRINTER_ALL_ACCESS} # Doesn't work with PRINTER_ACCESS_USE
handle = win32print.OpenPrinter(name, printdefaults)
level = 2
attributes = win32print.GetPrinter(handle, level)
#attributes['pDevMode'].Duplex = 1  #no flip
#attributes['pDevMode'].Duplex = 2  #flip up
attributes['pDevMode'].Duplex = 3   #flip over
win32print.SetPrinter(handle, level, attributes, 0)
win32print.GetPrinter(handle, level)['pDevMode'].Duplex
win32api.ShellExecute(0,'print','manual1.pdf','.','/manualstoprint',0)

你可以试试

win32print.SetDefaultPrinter("\\\\Server\Printer")

此方法接受String，而不是您试图传递给它的打印机对象。