我会尽我所能回答这个问题，根据帖子，有问题的缩进，使用正确的变量来获取值（stringlineofinteres而不是行，这是一个在循环），你的代码，最后没有行添加values到总数：

import os
import platform

pathwindows = os.environ['USERPROFILE'] + r"\Documents\Your_Wordle_Results.txt"
pathmac = r'/Mac/Users/%USEPROFILE%/Documents/Your_Wordle_Results.txt'

pathwindows="enum.txt"

isFileWindows = os.path.exists(pathwindows)
isFileMac = os.path.isfile(pathmac)

if isFileWindows == True:
    filepath=pathwindows
 
if isFileMac == True:
    filepath=pathmac
    

totalpoints1 = 0
totalpoints2 = 0
totalpoints3 = 0
totalpoints4 = 0
totalpoints5 = 0

with open(filepath, 'r') as fp:

    lineofinterest = fp.readlines()[2:100]
    stringlineofinterest = str(lineofinterest)
    print(*lineofinterest)
    for line in lineofinterest:
        print(line)

        startline = 22
        separation = 4
        value1 = (line[startline + separation * 0])
        totalpoints1 += int(value1)
        value2 = (line[startline + separation * 1])
        totalpoints2 += int(value2)
        value3 = (line[startline + separation * 2])
        totalpoints3 += int(value3)
        value4 = (line[startline + separation * 3])
        totalpoints4 += int(value4)
        value5 = (line[startline + separation * 4])
        totalpoints5 += int(value5)

# write the totals line here
with open(filepath,'a') as outfile:
    outfile.write("totals    xxxx")

print(totalpoints1, totalpoints2,totalpoints3,totalpoints4,totalpoints5)