您不应在color()内调用video = cv2.VideoCapture(1)。因此，您需要创建另一个由 “Inciar” 按钮调用的函数，然后创建video并在此函数内调用color()。
此外，lblVideo.after(...)之前的四行应该位于if块内。
以下是更新后的代码：

video = None

def color():
    global after_id
    ret, frame = video.read()
    if ret == True:
        frameHSV = cv2.cvtColor(frame,cv2.COLOR_BGR2HSV)
        frame=cv2.cvtColor(frame,cv2.COLOR_RGB2BGR)
        mask = cv2.inRange(frameHSV,azulBajo,azulAlto)
        contornos, hierarchy = cv2.findContours(mask, cv2.RETR_EXTERNAL,cv2.CHAIN_APPROX_SIMPLE)
        for c in contornos:
            area = cv2.contourArea(c)
            if area > 3000:
                M = cv2.moments(c)
                if (M["m00"]==0): M["m00"]=1
                x = int(M["m10"]/M["m00"])
                y = int(M['m01']/M['m00'])
                cv2.circle(frame, (x,y), 7, (0,255,0), -1)
                font = cv2.FONT_HERSHEY_SIMPLEX
                cv2.putText(frame, '{},{}'.format(x,y),(x+10,y), font, 0.75,(0,255,0),1,cv2.LINE_AA)
                nuevoContorno = cv2.convexHull(c)
                cv2.drawContours(frame, [nuevoContorno], 0, (255,0,0), 3)

        img=Image.fromarray(frame)
        image=ImageTk.PhotoImage(img)
        lblVideo.configure(image=image)
        lblVideo.image=image

    after_id = lblVideo.after(1, color)

def start_capture():
    global video
    if video is None:
        video = cv2.VideoCapture(1) # open the video cam
        lblVideo.place(x=0, y=0) # show the image label
        color()  # start the capture loop

def quitar():
    global video
    lblVideo.after_cancel(after_id)  # cancel the capture loop
    lblVideo.place_forget()
    video.release()
    video = None  # reset variable to None

boton2=Button(frame1,width=5, font = ('Arial',14, 'bold'), text='Inciar',
              bg='yellow',bd=5, command=start_capture)
boton2.place(x=85, y=210)
boton3=Button(frame1,width=5, font = ('Arial',14, 'bold'), text='Parar', 
              bg='yellow',bd=5, command=quitar)
boton3.place(x=290, y=210)