通过添加此条件，应用程序会检查请求的路径是否以“/static/"开头。如果是，则尝试直接从指定路径提供静态文件。这样，您的CSS和JS文件将得到正确的服务。

if path.startswith("/static/"):
                    # Serve static files directly
                    static_file_path = path.lstrip("/static/")
                    try:
                        with open(static_file_path, 'rb') as f:
                            content = f.read()
                        start_response("200 OK", [("Content-type", "text/css" if path.endswith(".css") else "text/javascript")])
                        return [content]
                    except FileNotFoundError:
                        start_response("404 Not Found", [("Content-type", "text/plain")])
                        return [b"404 Not Found"]

感谢Ramziya的回答，我想出了如何做到这一点，为此非常感谢！到目前为止，我只连接了CSS样式，但我已经知道如何用JS来做，我稍后会做！

from waitress import serve

def render_template(template_name, context={}):
 html_str=""
 with open(template_name, 'r') as f:
    html_str=f.read()
    html_str=html_str.format(**context)
 return html_str

def home(environ):
 return render_template('templates/index.html', context={})

def contact_us(environ):
 return render_template('templates/contact.html', context={})

def contact2(environ):
 return render_template('templates/2.html', context={})

def app(environ, start_response):
 path= environ.get("PATH_INFO")
 if path.startswith("/src/css/"):
    new_path = path.replace('/','',1)
    try:
        with open(new_path, 'rb') as f:
            content = f.read()
        start_response("200 OK", [("Content-type", "text/css")])
        return [content]
    except FileNotFoundError:
        start_response("404 Not Found", [("Content-type", "text/plain")])
        return [b"404 Not Found css/js"]
    

 if path == "/":
     page = home(environ)
 elif path == "/contact":
     page = contact_us(environ)
 elif path == "/contact/2":
     page = contact2(environ)
 else:
     page = render_template('templates/404.html', context={"path":path})
 page = page.encode("utf-8")

 start_response(
     f"200 OK", [
         ("Content-type", "text/html"),
     ]
 )
 return iter([page])

serve(app)